Publications

There are our publications (Engligh only) since 2007~. Please let us know if you want to see publications before 2006. Review/books (Japanese) were only shown in Japanese web site.

2019

  1. Negative Charge at Aspartate 151 is Important for Human Lens αA-crystallin Stability and Chaperone Function.
    Takata T, Toshiya M, Nakamura-Hirota T and Fujii N. Exp Eye Res. 182, 10-18 (2019).


2018

  1. Asp58 modulates lens αA-crystallin oligomer formation and chaperone function.
    Takata T, Nakamura-Hirota T, Inoue R, Morishima K, Sato N, Sugiyama M and Fujii N. FEBS J. (2018). in press.

  2. Identification of Isomeric Aspartate residues in βB2-crystallin from Aged Human Lens.

    Takata T, Murakami K, Toyama A and Fujii N. Biochimica et Biophysica Acta -Proteins and Proteomics (2018).    
    in press.


2017

  1. Identification of ᴅ-amino acid-containing peptides in human serum.
    Ha S, Kim I, Takata T, Kinouchi T,  Isoyama M, Suzuki M, Fujii N.  PLoS ONE 12(12): e0189972. (2017).


  1. Ferulic Acid Suppresses Amyloid β Production in the Human Lens Epithelial Cell Stimulated with Hydrogen Peroxide. Nagai N, Kotani S, Mano Y, Ueno A, Ito Y, Kitaba T, Takata T and Fujii N. Biomed Res Int, 2017:5343010. (2017).


  1. Isomeric replacement of a single aspartic acid induces a marked change in protein function: the example of ribonuclease A.
    Sakaue H, Kinouchi T, Fujii N, Takata T and Fujii N. ACS Omega. 2. 260-267 (2017). 


2016

  1. One-shot LC-MS/MS analysis of post-translational modifications including oxidation and deamidation of rat lens α- and β-crystallins induced by γ-irradiation.
    Kim I, Saito T, Fujii N, Kanamoto T and Fujii N. Amino Acids (2016). 

  2. Aggregation of Trp > Glu point mutants of human gamma-D crystallin provides a model for hereditary or UV-induced cataract.
    Serebryany E, Takata T, Erickson E, Schafheimer N, Wang Y, King JA. Protein science. 6. 1115-1128 (2016).

  3. New insight into the dynamical system of αB-crystallin oligomers.
    Inoue R, Takata T, Fujii N, Ishii K, Uchiyama S, Sato N, Oba Y, Wood K, Kato K, Fujii N and Sugiyama M. Scientific Reports. 6. 29208 (2016).

  4. Isomerization of Asp residues plays an important role in αA-crystallin dissociation.
    Takata T and Fujii N. FEBS J. 283 850-859 (2016).

2015

  1. Site specific oxidation of amino acid residues in rat lens γ-crystallin induced by low doseγ-irradiation.
    Kim I, Saito T, Fujii N, Kanamoto T, Chatake T and Fujii N. Biochemical and Biophysical Research Communications 466 622-628 (2015).

  2. Effect of Asp 96 isomerization on the properties of a lens αB-crystallin-derived short peptide.
    Takata T and Fujii N. J Pharm Biomed Anal. 116, 139-144 (2015).

  3. Immunohistochemical localization of D-β-aspartic acid-containing proteins in pterygium.
    Kaji Y, Oshika T, Nejima R, Mori S, Miyata K, Fujii N. J Pharm Biomed Anal. 116, 86-89 (2015).

  4. Interaction of αA-crystallin F71L mutant with wild type αA- and αB-crystallins by mammalian two hybrid assay.
    Ramkumar S, Thankappan B, Fujii N, Natarajaseenivasan K, Anbarasu K. Int J Biol Macromol. 76, 102-108 (2015).

  5. Real-time heterogeneous protein-protein interaction between αA-crystallin N-terminal mutants and αB-crystallin using quartz crystal microbalance (QCM).
    Ramkumar S, Fujii N, Sakaue H, Fujii N, Thankappan B, Kumari RP, Natarajaseenivasan K, Anbarasu K. Amino Acids. 47, 1035-1043 (2015).

  6. Rapid Survey of Four Asp Isomers in Disease-Related Proteins by LC-MS combined with Commercial Enzyme.
    Maeda H, Takata T,Fujii N,Sakaue H, Nirasawa S, Takahashi S, Sasaki H and Fujii N. Anal. Chem. 87, 561-568 (2015).

  7. αB- and βA3-crystallins containing D-Aspartic acids exist in a monomeri c state.
    Sakaue H, Takata T, Fujii N, Sasaki, H and Fujii N Biochimica et Biophysica Acta -Proteins and Proteomics,1854,1-9 (2015).


2014

  1. In vitro Amyloidogenic Peptides of Galectin-7: Possible Mechanism of Amyloidogenesis of Primary Localized Cutaneous Amyloidosis.
    Ono K, Fujimoto E, Fujimoto N, Akiyama M, Satoh T, Maeda H, Fujii N, Tajima S. J Biol Chem. 289, 29195-29207 (2014).

  2. Comparison of effect of gamma ray irradiation on wild-type and N-terminal mutants of αA-crystallin.
    Ramkumar S, Fujii N, Fujii N, Thankappan B, Sakaue H, Ingu K, Natarajaseenivasan K, Anbarasu K. Mol Vis. 20:1002-1016 (2014).

  3. Three cases of elastofibroma with immunohistochemical analysis of microfibrillar components.
    Uehara M, Matsuda K, Kato A, Sato S , Shimizu F, Ooatari1 M, Kai Y, Shimada H, Hatano Y, Okamoto O, Katagiri K, Sasaki T, Fujii N, Fujiwara S. Eur J Dermatol (2014).

  4. Effects of carotenoids on damage of biological lipids induced by gamma irradiation.
    Saito T. and Fujii N. Radiat. Phys. Chem. 98, 57-63 (2014).

2013

  1. Simultaneous ultraviolet-B induced photo-oxidation of tryptophan/tyrosine and racemization of neighboring aspartyl residues in peptides.
    Cai S, Fujii N, Saito T, and Fujii N. Free Radical Biology & Medicine 65, 1037-1046 (2013).

  2. Antimicrobial and Antibiofilm Activity of Designed and Synthesized Antimicrobial Peptide, KABT-AMP.
    Thankappan B, Jeyarajan S, Sakaue H, Anbarasu K, Natarajaseenivasan K. & Fujii N. Appl Biochem Biotechnol. 170, 1184-1193 (2013).

  3. Galectin-7 and actin are components of amyloid deposit of localized cutaneous amyloidosis.
    Miura, Yoshinori; Harumiya, Satoru; Ono, Koji; Fujimoto, Eita; Akiyama, Minoru; Fujii, Noriko; Kawano, Hiroo; Wachi, Hiroshi; Tajima, Shingo. Experimental Dermatology 22, 36-40 (2013).

  4. Kinetics of isomerization and inversion of aspartate 58 of αA-crystallin peptide mimics under physiological conditions.
    Kenzo Aki, Norihiko Fuji and Noriko Fujii. PLoS One. 8 (3):e58515. (2013).

2012

  1. A rapid comprehensive LC-MS based survey of the Asp isomers in crystallins from human cataract lenses.
    Fujii N, Sakaue H. Sasaki H. and *Fujii N. J. Biol Chem. VOL.287, NO.47, PP.39992-40002, (2012).

  2. Chronic exposure to low frequency noise at moderate levels causes impaired balance in mice.
    Tamura H, Ohgami N, Yajima I, Iida M, Ohgami K, Fujii N, Itabe H, Kusudo T, Yamashita H, Kato M. PLoS One. 7 (6):e39807. (2012).

  3. A P39R mutation at the N-terminal domain of human αB-crystallin regulates its oligomeric state and chaperone-like activity.
    Numoto N, Kita A, Fujii N, Miki K., Biochem Biophys Res Commun.,425,:601-606 (2012).

  4. Co-localisation of advanced glycation end products and D-β-aspartic acid-containing proteins in gelatinous drop-like corneal dystrophy.
    Kaji Y, Oshika T, Takazawa Y, Fukayama M, Fujii N., Br J Ophthalmol, 96(8):1127-31. (2012).

  5. Structural features of isomerizable aspartyl residues in human α-crystallins.
    Ken-ichi Shimizu, Akiko Kita, Noriko Fujii, and Kunio Miki, Molecular Vision, 18, 1823-1827 (2012).

  6. Characterization of an antibody that recognizes peptides containing D-β-aspartyl residues.
    Kenzo Aki, Norihiko Fujii, Takeshi Saito and Noriko Fujii, Molecular Vision, 18,996-1003(2012).

2011

  1. Characterization and Radio-resistant Function of Manganese Superoxide Dismutase of Rubrobacter radiotolerans.
    H. Terato, K. Suzuki, N. Nishioka, A. Okamoto, Y. Shimazaki-Tokuyama, Y. Inoue and T. Saito J. Radiat. Res. 22, 735-742. (2011).

  2. Simultaneous stereoinversion and isomerization at the Asp-4 residue in βB2-crystallin from the aged human eye lenses.
    Norihiko Fujii, Takeshi Kawaguchi, Hiroshi Sasaki and Noriko Fujii. Biochemistry 50, 8628-8635 (2011).

  3. UV B-irradiation enhances the racemization and isomerizaiton of aspartyl residues and production of Nε-carboxymethyl lysine (CML) in keratin of skin.
    Yuhei Mori, Kenzo Aki, Katsunori Kuge, Natsuko Yamanaka, Shingo Tajima, Yuichi Kaji, Ryoji Nagai, Hanako Yoshii, Naoki Yamamoto, Norihiko Fujii, Masami Watanabe, Tadatoshi Kinouchi and Noriko Fujii. J. Chromatogr. B 879, 3303-3309 (2011).

  4. Substrate stereoselectivity of mammalian D-aspartyl endopeptidase.
    Tadatoshi Kinouchi, Norihiko Fujii and Noriko Fujii. J. Chromatogr. B Analyt Technol Biomed Life Sci. 879, 3349-3352. (2011).

  5. Determination of rate constants for beta-linkage isomerization of three specific aspartyl residues in recombinant human alpha A-crystallin protein by reversed-phase HPLC.
    Yutaka Sadakane, Noriko Fujii, Kazuya Nakagomi . J Chromatogr. B 879, 3240-3246 (2011).

2010

  1. Influence of Lβ-, Dα-, and Dβ-Asp isomers of the Asp-76 residue on the properties of αA-crystallin 70-88 peptide.
    Noriko Fujii, Norihiko Fujii, Masashi Kida and Tadatoshi Kinouchi. Amino Acids 39, 1393-1399 (2010).

  2. Differentiation and Semiquantitative analysis of an isoaspartic acid in human alpha -crystallin by Post Source Decay on Curved Field Reflectron.
    Yuzo Yamazaki, Norihiko Fujii, Yutaka Sadakane and Noriko Fujii. Anal. Chem. 82, 6384－6394 (2010).

  3. The Protective Effects of Metallothionein I and II against Metal- or Ultraviolet Radiation-Induced Damages in Cultured Lens Epithelial Cells.
    Saito T, Tezuka T, Konno R. and Fujii N. Jpn J. Ophthalmol. 54, 486-493 (2010).

  4. Accumulation of D-β-Aspartic acid-containing proteins in age-related ocular diseases.
    Yuichi Kaji, Tetsuro Oshika, Yutaka Takazawa, Masashi Fukayama, Noriko Fujii. Chemistry and Biodiversity 7, 1364-1370 (2010).

  5. SANS and SAXS Observations of Abnormal Aggregation of alpha-Crystallin.
    Masaaki Sugiyama, Norihiko Fujii, Yukio Morimoto, Keiji Itoh, Kazuhiro Mori, Toshiharu Fukunaga, and Noriko Fujii. Chemistry and Biodiversity 7, 1380-1388 (2010).

  6. Influence of oxidative stress on D-aspartyl endopeptidase activity.
    Tadatoshi Kinouchi, Takahiko Shimizu, Takuji Shirasawa and Noriko Fujii. Chemistry and Biodiversity 7, 1398-1402 (2010).

  7. Structural consideration of mammalian D-aspartyl endopeptidase.
    Tadatoshi Kinouchi and Noriko Fujii. Chemistry and Biodiversity 7, 1403-1407 (2010).

  8. Oxidative Stress Indces the Formation of D-Aspartyl Residues in the Elastin Mimic Peptide.
    Katusnori Kuge, Keiko Kitamura, Koichi Nakaoji, Kazuhiko Hamada, Norihiko Fujii, Takeshi Saito, Noriko Fujii. Chemistry and Biodiversity 7, 1408-1412 (2010).

  9. Genome-wide expression changes in Saccharomyces cerevisiae in response to high-LET ionizing radiation.
    S. Mizukami-Murata, H. Iwahashi, S. Kimura, K. Nojima, Y. Sakurai, T. Saitou, N. Fujii, Y. Murata, S. Suga, K. Kitagawa, K. Tanaka, S. Endo and M. Hoshi Appl. Biochem. Biotechnol. 162, 855-870.(2010).

2009

  1. Localization of D-β-Aspartyl Residue-Containing Proteins in Various Tissues.
    Motoie R, Fujii N,* Tsunoda S, Nagata K, Shimo-oka T, Kinouchi T, Fujii N, Saito T and Ono K. Int. J. Mol. Sci. 10, 1999-2009 (2009).

  2. A Simple and Rapid Method for Measurement of (10)B-para-Boronophenylalanine in the Blood for Boron Neutron Capture Therapy Using Fluorescence Spectrophotometry.
    Kashino G, Fukutani S, Suzuki M, Liu Y, Nagata K, Masunaga S, Maruhashi A, Tanaka H, Sakurai Y, Kinashi Y, Fujii N, Ono K. Radiat Res (Tokyo). 50, 377-382 (2009).

  3. Tryptophanase-Catalyzed l-Tryptophan Synthesis from d-Serine in the Presence of Diammonium Hydrogen Phosphate.
    Shimada A, Ozaki H., Saito T. and Fujii N. Int. J. Mol. Sci. 10, 2578-2590 (2009).

  4. Immunohistochemical localization of D-β-aspartic acid-containing proteins in climatic droplet keratopathy.
    Kaji Y, Oshika T, Takazawa Y, Fukayama M, Fujii N. Br. J. Ophthalmol. 93, 977-979 (2009).

  5. Immunohistochemical localization of D-beta-aspartic acid in pinguecula.
    Kaji Y, Oshika T, Okamoto F, Fujii N. Br J Ophthalmol. 93, 974-976 (2009).

  6. Screening system for D-Asp-containing proteins using D-aspartyl endopeptidase and two-dimensional gel electrophoresis.
    Sakai-Kato K, Kinouchi T, Fujii N, Imai K, Utsunomiya-Tate N. Amino Acids 36, 125-129 (2009).

2008

  1. Study of subunit interactions of alpha A- and alpha B- crystallins and the effects of gamma-irraditation on their interactions by surface plasmon resonance.
    Fujii N. Hisano T. and Fujii N. Biochim. Biophys. Acta 1784, 1507-1513 (2008).

  2. Differential rate constants of racemization of aspartyl and asparaginyl residues in human alpha A-crystallin mutants.
    Nakamura T., Sakai M., Sadakane Y., Haga T., Goto Y., Kinouchi T., Saito T, and Fujii N. Biochim. Biophys. Acta 1784, 1192-1199 (2008).

  3. Structural evolution of human recombinant alpha B-crystallin under UV irradiation.
    Sugiyama M., Fujii N., Morimoto Y., Kurabayashi S., Vigild, M., Sato T., Nakagawa T., Ito K., Mori K. and Fukunaga T. Biomacromolecules 9, 431-434. (2008).

  4. Effects of UV irradiation on ESR and optical properties of Cu(II) ions in organic solid solutions containing CuCl at 77 K.
    Y.M. Hase, Y. Nakano and T. Saito. The Open Magnetic Resonance Journal 1, 81-87. (2008).

2007

  1. Localization of D-beta-aspartic acid-containing proteins in human eyes.
    Kaji Y, Oshika T, Takazawa Y, Fukayama M, Takata T, Fujii N. Invest. Ophthalmol. Vis. Sci. 48, 3923-3927. (2007).

  2. Conformation-dependent racemization of aspartyl residues in peptides.
    Kuge K., Brack A., and Fujii N. Chem. Eur. 13, 5617-5621. (2007).

  3. Shape of alpha-Crystallin Analyzed by Small-Angle Neutron Scattering.
    Masaaki Sugiyama M., Fujii N., Morimoto Y., Otomo T., Takata T., Misawa M, Annaka M., Mori K., Sato T., Kurabayashi S. and Fukunaga T. J. Appl. Crystal. 40, 200-204. (2007).

  4. Age related changes of alpha-crystallin aggregate in human lens.
    Fujii N, Shimmyo Y, Sakai M, Sadakane Y, Nakamura T, Kinouchi T, Morimoto Y, Goto Y and Lampi K. Amino Acids. 32, 87-94. (2007).

  5. Isolation and characterization of mammalian D-aspartyl endopeptidase.
    Kinouchi T, Nishio H, Nishiuchi Y, Tsunemi M, Takada K, Hamamoto T, Kagawa Y, Fujii N. Amino Acids. 32, 79-85. (2007).

  6. Differential susceptibility of alpha A- and alpha B-crystallin to gamma-ray irradiation.
    Fujii N., Nakamura T, Sadakane Y, Saito T, and Fujii N, Biochim. Biophys. Acta. 1774, 345-350. (2007).


Review/Books (English)

  1. "The Importance of the Idea of“Parachirality” in Life Science.
    Noriko Fujii, Norihiko Fujii, Takumi Takata  and Hiroaki Sakaue. Chapter 6, “ADVANCES IN ASYMMETRIC AUTOCATALYSIS AND RELATED TOPICS (the'Work") edited by Gyula Palyi, Robert Kurdi and Claudia Zucchi (the "Editor”). Chapter 6, pp119-131. Elsevier Inc. (2017) ISBN: 978-0-12-812824-4.

  2. D-Amino acid residues in proteins related to aging and age-related diseases and a new analysis of the isomers in proteins.
    Noriko Fujii, Takumi Takata, Norihiko Fujii, Kenzo Aki and Hiroaki Sakaue. pp 241- 254“D-Amino acids: Physiology, Metabolism, and Application” edited by Yoshimura T, Nishikawa T and Homma H. Springer, (2016) ISBN: 978-4-431-56075-3.

  3. Isomerization of aspartyl residues in crystallins and its influence upon cataract.
    Noriko Fujii, Takumi Takata, Norihiko Fujii , Kenzo Aki. Biochimica et Biophysica Acta 1860,183-191 (2016).

  4. Quantitative analysis of isomeric (l-α-, l-β-, d-α-, d-β-) aspartyl residues in proteins from elderly donors.
    Noriko Fujii,Takumi Takata, Norihiko Fujii Journal of Pharmaceutical and Biomedical Analysis, 116,25-33 (2015).

  5. Emerging issues in radiogenic cataracts and cardiovascular disease.
    Hamada N, Fujimichi Y, Iwasaki T, Fujii N, Furuhashi M, Kubo E, Minamino T, Nomura T and Sato H. J. Rad. Res., 1－16 (2014) Review Article.

  6. Non-Enzymatic Post-Translational Modifications in the Development of Age-Related Macular Degeneration. 
    Yuichi Kaji, Tetsuro Oshika and Noriko Fujii. pp 73-82 Chapter 4 in Age Related Macular Degeneration - The Recent Advances in Basic Research and Clinical Care. Edited by Gui-Shuang Ying. InTech, January, 2012, ISBN #978-953-307-864-9.

  7. Analysis of D-β-aspartyl Isomers at Specific Sites in Proteins.
    Noriko Fujii and Norihiko Fujii. Methods in Molecular Biology , Vol. 794 pp325-335 Chapter 22: Unnatural Amino Acids, Pollegioni, Loredano; Servi, Stefano (Eds.) Springer New York, 2012, ISBN 978-1-61779-330-1.

  8. D-amino-acid-containing proteins in living body.
    Noriko Fujii, Kenzo Aki and Norhiko Fujii.  “The Soai Reaction and Related topic” edited by Gyula Pály, Claudia Zucchi, Luciano Caglioti. Accademia Nazionale di Scienze Lettere e Arti Modena, Italy. ISBN 978-88-6462-130-2 Chapter 2, pp35-52 (2012) (Total 376 pages).

  9. Abnormal protein aggregation due to the presence of D-aspartyl residues in cataractous lenses.
    Noriko Fujii, Norihiko Fujii and Masaaki Sugiyama,Trans.Mat.Res.Soc.Japan, 37,131-134 (2012).

  10. D-Amino acids in aged proteins: analysis and biological relevance.
    Noriko Fujii, Yuichi Kaji and Norihiko Fujii. J. Chromatogr. B 879, 3141-3147 (2011).

  11. Pathological Role of D-amino Acid-Containing Proteins and Advanced Glycation End Products in the Development of Age-Related Macular Degeneration.
    Yuichi Kaji, Tetsuro Oshika, Yutaka Takazawa, Masashi Fukayama, Noriko Fujii. Anti-Aging Medicine 7 (10) : 107-111, 2010 review.

  12. Collapse of Homochirality of Amino Acids in Protein from Various Tissues During Aging.
    Noriko Fujii, Yuichi Kaji, Norihiko Fujii, Ryota Motoie, Yuhei Mori and Tadatoshi Kinouchi. Chemistry and Biodiversity 7, 1389-1397 (2010).

  13. Detection of the specific D-aspartic acid residues in protein.
    Fujii N. and Takata T. “D-Amino Acids: A New Frontier in Amino Acid and Protein Rsearch-practical Methods and Protocol”s. edited by Konno R, Bruckner H, D'Aniello, Fujii N and Homma H. Nova Science Publishers, New York pp355-367 (2007).